An Army virologist found traces of Ebola virus in patient samples in West Africa seven years before the largest, deadliest Ebola outbreak took the world by surprise in Guinea, Liberia and Sierra Leone.
In 2006, Dr. Randal J. Schoepp, chief of the Applied Diagnostics Department in the Diagnostic Systems Division at the U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID), was working in Sierra Leone at the Kenema Government Hospital in Eastern Province.
Biosecurity Capacity Building
As part of a collaboration between USAMRIID and Tulane University scientists, Schoepp was supporting research to develop and refine Lassa fever diagnostic tests and build host-country diagnostic capacity.
Lassa is a hemorrhagic fever illness that is endemic to Africa. The number of West African Lassa virus infections is 100,000 to 300,000 a year with about 5,000 deaths, according to the Centers for Disease Control and Prevention.
“My interest has always been arthropod-borne diseases — in other words, mosquito-borne and tick-borne viruses, and hemorrhagic fever viruses,” Schoepp said during a recent DoD News interview at USAMRIID.
“One reason I was interested in Sierra Leone is because, for those of us who work with hemorrhagic fevers … this is the only place you can study them because you know they’re going to show up and you know where they’re going to be,” the virologist said.
As the work continued in Sierra Leone, the scientists found that, of the 500 to 700 samples a year submitted to the Kenema Government Hospital Lassa Diagnostic Lab from Sierra Leone, Liberia and Guinea, only 30 percent to 40 percent were actually Lassa. Schoepp said he got interested in the 60 percent to 70 percent that weren’t Lassa.
The aim of his study, he explained, was to find out which other viruses caused serious illnesses in the region and to help medical and technical personnel there learn how to detect the illnesses.
The samples Schoepp looked at already had been screened for Lassa and malaria, and he and his colleagues ended up with about 400 samples, taken from 2006 to 2008, that represented 253 patients, he said.
In these samples he looked for other arthropod-borne viruses — “dengue, Rift Valley fever, West Nile virus, yellow fever virus, all the ones you would expect to see in Africa,” he said — as well as hemorrhagic fever viruses such as Crimean Congo hemorrhagic fever, Marburg, Ebola and others.
Looking For Lassa, Finding Ebola
Out of all that, he said, the most significant finding “was that 8.6 percent of the samples had the earliest antibodies to Ebola,” called immunoglobulin M, or IgM, antibodies.
IgM is the first antibody to be made by the body to fight a new infection, Schoepp said, “so if you find IgM antibodies it tells you that you’re very close to the original infection.”
Looking further into the Ebola antibodies with the plaque reduction neutralization test, which many scientists consider the “gold standard” for detecting and measuring antibodies that can neutralize many disease-causing viruses, Schoepp saw that most of the Ebola antibodies were against the Zaire strain.
Ebola Zaire is the most virulent of the virus’s five strains, and the one that is now causing the West African outbreak.
In a region supposedly untouched by Ebola except for a single case of the Tai Forest strain reported in Cote d’Ivoire in 1994, Schoepp said, this was big news that at the time could have been unwelcome in the three countries.
“I spent over a year going back to Sierra Leone, talking to the regional medical officers, talking to the ministry, making them understand that this is what we found,” in a careful process of medical diplomacy, Schoepp said.
Afterward, in August 2013, he submitted a scientific paper about the West African Ebola finding to CDC’s Emerging Infectious Diseases journal. After nearly a year and reviews by two sets of scientists, the final reviewer told Schoepp, “I don’t believe there is Ebola virus in West Africa.”
A week later, Schoepp said, the West African Ebola outbreak was announced to the world and, after an email from Schoepp to the journal editor, “Undiagnosed Acute Viral Febrile Illnesses, Sierra Leone,” was published in July 2014.
“To me, it means that there is more Ebola out in the world than you would know by past outbreaks or by other evidence,” Schoepp said, discussing the paper’s results. “If you look for it, you have a very good chance of finding it.”
Diagnostics, he added, is the basis of everything.
“We set the stage for others to come in and do their therapeutics, their antivirals, their vaccines,” Schoepp said. “Knowing what’s there is one thing, and being able to do something about what’s there is another thing. So diagnostics gives the epidemiologists, immunologists and the therapeutic people something to do.”
In a time when globalization spreads diseases farther and faster than ever, Schoepp said, it’s a good time to be a virologist.
“Every time you think everything has happened, [severe acute respiratory syndrome] pops up or [Middle East respiratory syndrome-corona virus] pops up or Ebola pops up,” he said, adding that he often describes a virologist as being like a fireman. “Somebody says ‘fire’ and a fireman runs toward it,” Schoepp said. “Somebody says ‘disease’ and a virologist runs toward it.”
Schoepp recently returned from a recent trip to Liberia and Sierra Leone, where he spent twelve weeks helping to set up an Ebola testing lab and training local personnel to run Ebola diagnostic tests on clinical samples. Schoepp is part of a USAMRIID team that has been in West Africa since March.
Article by Cheryl Pellerin, DoD News, Defense Media Activity, adapted for context and format by CBRNe Central.